三疣梭子蟹几丁质酶基因(PtCht6)的克隆及其在免疫中的功能分析     

宋柳  吕建建  王磊  孙东方  刘萍 《海洋与湖沼》2019,50(5):1080-1090

几丁质酶(chitinase)在甲壳动物的蜕皮、消化和免疫等很多生物学过程中发挥重要功能,为深入探讨几丁质酶的免疫防御机制,本实验利用RACE技术克隆了三疣梭子蟹(Portunus trituberculatus)PtCht6基因。该基因cDNA全长2736bp,开放阅读框(ORF)2103bp,编码700个氨基酸,具有几丁质酶GH18家族典型特征。利用实时荧光定量技术分析PtCht6在三疣梭子蟹不同组织、不同蜕皮阶段、不同病原感染以及低盐胁迫(11)下的表达特征,结果显示:PtCht6在各组织中均有表达且在肝胰腺中的表达量最高;在不同蜕皮时期的肝胰腺中,其表达量由蜕皮后期(A/B)、蜕皮间期(C)、蜕皮前期(D)依次递减(P0.05);人工感染WSSV和副溶血弧菌后.PtCht6的表达量在肝胰腺中分别于12h、72h达到最大值,在血细胞中均于12h达到最大值,且相较于对照组,整体显著上调表达(P0.05);低盐胁迫能够显著抑制该基因的表达,最高抑制73倍(P0.05)。同时发现,低盐环境下感染WSSV后,该基因达到峰值的时间明显延迟(至少延迟12h.P0.05)。该研究结果预示PtCht6作为免疫因子参与三疣梭子蟹的病原防御,且低盐胁迫在一定程度上抑制了该基因的免疫功能。  相似文献   

A study of coral reef resilience and implications of adaptive management and rehabilitation in Khanh Hoa Province, Vietnam     

VO Si Tuan  HUA Thai Tuyen  PHAN Kim Hoang 《海洋学报(英文版)》2019,38(1):112-117

The Province of Khanh Hoa, Vietnam, is located in the western South China Sea and boasts a shoreline of 385 km and many islands. Previous studies have indicated the extreme diversity and abundance of coral reefs in its waters as compared with other coastal provinces of Vietnam. A study on the resilience of coral reefs against increased surface water temperature and anthropogenic impact is conducted at 19 reef sites in 2015. At each site, a series of parameters (e.g., coral covers, genus diversity, and coral recruitment, substratum heterogeneity, depth, water exchange level, and sediment deposit and water temperature) are measured quantitatively or semi-quantitatively. The measured data are rated based on the relationship between the parameter values and coral susceptibility; the consideration that reef health reflects the biological capability to adapt to environmental changes and the recruitment potential if bleached; and positive or negative influences of physical factors in the mitigation of thermal stress and protecting corals from bleaching. A cumulative analysis enables researchers to divide the studied reefs into four categories based on varying levels of reef health to support resilience, recovery, and vulnerability in the case of increased water temperature. Relevant management interventions for each category and other supporting activities are suggested to enhance management effectiveness and to plan the rehabilitation of coral reefs for biodiversity conservation and touristic development, taking into account the involvement of related stakeholders.  相似文献   

Inference of in situ stress from thermoporoelastic borehole breakouts based on artificial neural network     

Hua Zhang  Shunde Yin 《国际地质力学数值与分析法杂志》2019,43(16):2493-2511

The determination of in situ stresses is very important in petroleum engineering. Hydraulic fracturing is a widely accepted technique for the determination of in situ stresses nowadays. Unfortunately, the hydraulic fracturing test is time-consuming and expensive. Taking advantage of the shape of borehole breakouts measured from widely available caliper and image logs to determine in situ stress in petroleum engineering is highly attractive. By finite element modeling of borehole breakouts considering thermoporoelasticity, the authors simulate the process of borehole breakouts in terms of initiation, development, and stabilization under Mogi-Coulomb criterion and end up with the shape of borehole breakouts. Artificial neural network provides such a tool to establish the relationship between in situ stress and shape of borehole breakouts, which can be used to determine in situ stress based on different shape of borehole breakouts by inverse analysis. In this paper, two steps are taken to determine in situ stress by inverse analysis. First, sets of finite element modeling provide sets of data on in situ stress and borehole breakout measures considering the influence of drilling fluid temperature and pore pressure, which will be used to train an artificial neural network that can eventually represent the relationship between the in situ stress and borehole breakout measures. Second, for a given measure of borehole breakouts in a certain drilling fluid temperature, the trained artificial neural network will be used to predict the corresponding in situ stress. Results of numerical experiments show that the inverse analysis based on finite element modeling of borehole breakouts and artificial neural network is a promising method to determine in situ stress.  相似文献   

Current-induced scour beneath initially elevated subsea pipelines     

《Applied Ocean Research》2019

When a subsea pipeline is laid on an uneven seabed, certain sections may have an initial elevation with respect to the far-field seabed, e_o, and thus potentially affecting the on-bottom stability of the pipeline. This paper focuses on quantifying the effects of the upstream dimensionless seabed shear stress, θ_∞, and Reynolds number, Re, on: (1) the maximum dimensionless seabed shear stress beneath the pipe, θ_max, to be compared to the critical shear stress in order to determine whether scour would occur and progress towards an equilibrium state; and, (2) the dimensionless equilibrium scour depth beneath the pipe, S_eq/D. Using a 2-D Reynolds averaged Navier-Stokes (RANS) approach along with the k-ω Shear Stress Transport (SST) turbulence model, a parametric study involving 243 computational fluid dynamics (CFD) simulations was conducted. The simulation results were used to develop a closed-form equation for the prediction of θ_max. Subsequently, experimental measurements of S_eq/D have been compiled from published literature, to develop a new closed-form equation for the prediction of S_eq/D with a high correlation to the experimental data. In summary, we present two closed-form equations for the prediction of θ_max and S_eq/D for pipelines with an initial e_o/D, which are applicable for both clear-water and live-bed conditions. The effects of θ_∞ and Re have been included, albeit Re having a small influence as compared to the other parameters.  相似文献   

Integrating isolated and riparian wetland modules in the PHYSITEL/HYDROTEL modelling platform: model performance and diagnosis          下载免费PDF全文

Maxime Fossey  Alain N. Rousseau  Fatima Bensalma  Stéphane Savary  Alain Royer 《水文研究》2015,29(22):4683-4702

Mathematical modelling is a well‐accepted framework to evaluate the effects of wetlands on stream flow and watershed hydrology in general. Although the integration of wetland modules into a distributed hydrological model represents a cost‐effective way to make this assessment, the added value brought by landscape‐specific modules to a model's ability to replicate basic hydrograph characteristics remains unclear. The objectives of this paper were the following: (i) to present the adaptation of PHYSITEL (a geographic information system) to parameterize isolated and riparian wetlands; (ii) to describe the integration of specific isolated wetland and riparian wetland modules into HYDROTEL, a distributed hydrological model; and (iii) to evaluate the performance of the updated modelling platform with respect to the capacity of replicating various hydrograph characteristics. To achieve this, two sets of simulations were performed (with and without wetland modules), and the added value was assessed at three river segments of the Becancour River watershed, Quebec, Canada, using six general goodness‐of‐fit indicators and 14 water flow criteria. A sensitivity analysis of the wetland module parameters was performed to characterize their impact on stream flows of the modelled watershed. Results of this study indicate the following: (i) integration of specific wetland modules can slightly increase the capacity of HYDROTEL to replicate basic hydrograph characteristics; and (ii) the updated modelling platform allows for the explicit assessment of the impact of wetlands (e.g. typology and location) on watershed hydrology. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

A smoothed cap model for variably saturated soils and its robust numerical implementation          下载免费PDF全文

P. Gamnitzer  G. Hofstetter 《国际地质力学数值与分析法杂志》2015,39(12):1276-1303

This paper deals with the numerical implementation of a cap model for unsaturated soils. It provides a brief review of existing cap model approaches, based on which an improved model formulated in terms of generalised effective stress and matric suction is derived and described in detail. Although the proposed model is a multisurface plasticity model, it can efficiently be implemented using only single‐surface projections because of the smoothness of the model, which is obtained by construction. Numerical algorithms are provided for these single‐surface stress projections, using a single‐equation approach whenever possible. The robustness of the utilised single‐equation approaches is enhanced by proposing problem‐fitted start‐up procedures based on investigations of the nonlinear projection equations. A comparison of the model response with extensive material test data is used to validate the model and to demonstrate the robust application of the approach to silty sands and low to medium plasticity clays. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

The effective stress concept and the evaluation of changes in pore air pressure under jacketed isotropic compression tests for dry sand based on the 2‐phase porous theory          下载免费PDF全文

Sayuri Kimoto  Fusao Oka  Yasuhiro Morimoto 《国际地质力学数值与分析法杂志》2017,41(18):1894-1907

The effective stress concept for solid‐fluid 2‐phase media was revisited in this work. In particular, the effects of the compressibility of both the pore fluid and the soil particles were studied under 3 different conditions, i.e., undrained, drained, and unjacketed conditions based on a Biot‐type theory for 2‐phase porous media. It was confirmed that Terzaghi effective stress holds at the moment when soil grains are assumed to be incompressible and when the compressibility of the pore fluid is small enough compared to that of the soil skeleton. Then, isotropic compression tests for dry sand under undrained conditions were conducted within the triaxial apparatus in which the changes in the pore air pressure could be measured. The ratio of the increment in the cell pressure to the increment in the pore air pressure, m, corresponds to the inverse of the B value by Bishop and was obtained during the step loading of the cell pressure. In addition, the m values were evaluated by comparing them with theoretically obtained values based on the solid‐fluid 2‐phase mixture theory. The experimental m values were close to the theoretical values, as they were in the range of approximately 40 to 185, depending on the cell pressure. Finally, it was found that the soil material with a highly compressible pore fluid, such as air, must be analyzed with the multi‐phase porous mixture theory. However, Terzaghi effective stress is practically applicable when the compressibilities of both the soil particles and the pore fluid are small enough compared to that of the soil skeleton.  相似文献   

Energy partitioning over a semi‐arid shrubland in northern China          下载免费PDF全文

X. Jia  T. S. Zha  J. N. Gong  B. Wu  Y. Q. Zhang  S. G. Qin  G. P. Chen  W. Feng  S. Kellomäki  H. Peltola 《水文研究》2016,30(6):972-985

During the last decade, the widely distributed shrublands in northern China have shown significant signs of recovery from desertification, the result of widespread conservation practices. However, to support the current efforts in conservation, more knowledge is needed on surface energy partitioning and its biophysical controls. Using eddy‐covariance measurements made over a semi‐arid shrubland in northwest China in 2012, we examined how surface energy‐balance components vary on diurnal and seasonal scales, and how biophysical factors control bulk surface parameters and energy exchange. Sensible heat flux (H) exceeded latent heat flux (λE) during most of the year, resulting in an annual Bowen ratio (β, i.e. H/λE) of 2.0. λE exceeded H only in mid‐summer when frequent rainfall co‐occurred with the seasonal peak in leaf area index (LAI). Evapotranspiration reached a daily maximum of 3.3 mm day^?1, and summed to 283 mm yr^?1. The evaporative fraction (EF, i.e. λE/R_n), Priestley–Taylor coefficient (α), surface conductance (g_s) and decoupling coefficient (Ω) were all positively correlated with soil water content (SWC) and LAI. The direct enhancement of λE by high vapour pressure deficit (VPD) was buffered by a concurrent suppression of g_s. The g_s played a direct role in controlling EF and α by mediating the effects of LAI, SWC and VPD. Our results highlight the importance of adaptive plant responses to water scarcity in regulating ecosystem energy partitioning, and suggest an important role for revegetation in the reversal of desertification in semi‐arid areas. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

A 3D discrete FEM iterative algorithm for solving the water pipe cooling problems of massive concrete structures          下载免费PDF全文

Jing Cheng  T. C. Li  Xiaoqing Liu  L. H. Zhao 《国际地质力学数值与分析法杂志》2016,40(4):487-508

Water pipe cooling has been widely used for the temperature control and crack prevention of massive concrete structures such as high dams. Because both under‐cooling and over‐cooling may reduce the efficiency of crack prevention, or even lead to great harm to structures, we need an accurate and robust numerical tool for the prediction of cooling effect. Here, a 3D discrete FEM Iterative Algorithm is introduced, which can simulate the concrete temperature gradient near the pipes, as well as the water temperature rising along the pipes. On the basis of the heat balance between water and concrete, the whole temperature field of the problem can be computed exactly within a few iteration steps. Providing the pipe meshing tool for building the FE model, this algorithm can take account of the water pipe distribution, the variation of water flow, water temperature, and other factors, while the traditional equivalent algorithm based on semi‐theoretical solutions can only solve problems with constant water flow and water temperature. The validation and convergence are proved by comparing the simulated results and analytical solutions of two standard second‐stage cooling problems. Then, a practical concrete block with different cooling schemes is analyzed and the influences of cooling factors are investigated. In the end, detailed guidance for pipe system optimization is provided. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

Implicit integration under mixed controls of a breakage model for unsaturated crushable soils          下载免费PDF全文

Yida Zhang  Giuseppe Buscarnera 《国际地质力学数值与分析法杂志》2016,40(6):887-918

This paper discusses a series of stress point algorithms for a breakage model for unsaturated granular soils. Such model is characterized by highly nonlinear coupling terms introduced by breakage‐dependent hydro‐mechanical energy potentials. To integrate accurately and efficiently its constitutive equations, specific algorithms have been formulated using a backward Euler scheme. In particular, because implementation and verification of unsaturated soil models often require the use of mixed controls, the incorporation of various hydro‐mechanical conditions has been tackled. First, it is shown that the degree of saturation can be replaced with suction in the constitutive equations through a partial Legendre transformation of the energy potentials, thus changing the thermomechanical state variables and enabling a straightforward implementation of a different control mode. Then, to accommodate more complex control scenarios without redefining the energy potentials, a hybrid strategy has been used, combining the return mapping scheme with linearized constraints. It is shown that this linearization strategy guarantees similar levels of accuracy compared with a conventional strain–suction‐controlled implicit integration. In addition, it is shown that the use of linearized constraints offers the possibility to use the same framework to integrate a variety of control conditions (e.g., net stress and/or water‐content control). The convergence profiles indicate that both schemes preserve the advantages of implicit integration, that is, asymptotic quadratic convergence and unconditional stability. Finally, the performance of the two implicit schemes has been compared with that of an explicit algorithm with automatic sub‐stepping and error control, showing that for the selected breakage model, implicit integration leads to a significant reduction of the computational cost. Such features support the use of the proposed hybrid scheme also in other modeling contexts, especially when strongly nonlinear models have to be implemented and/or validated by using non‐standard hydro‐mechanical control conditions. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献